English
聯系我們
網站地圖
郵箱
舊版回顧



科學家繪制造血干細胞擴增組織的3D轉錄組圖譜

文章來源:動物研究所   發布時間:2019-05-10  【字號:     】  

  血液系統中貯藏著一種具有自我更新、分化成各種血細胞潛能的成體干細胞,稱為造血干細胞,它能夠維持機體長久造血和組織穩態。造血干細胞移植是惡性血癌的有效治療手段,但干細胞來源不足成為限制該治療廣泛應用的瓶頸。因此,造血干細胞的發育,尤其是造血干細胞擴增的研究備受關注。然而,現在的研究主要集中在特定細胞群體或關鍵因子的“線性化”或“平面化”解析,缺乏全面“立體化”的分析。從系統生物學角度解析擴增性造血器官,將為造血干細胞擴增的機制研究提供更多的啟示。鑒于造血發育過程的高度保守性,中國科學院動物研究所劉峰研究組及合作者以斑馬魚尾部造血組織(在功能上類似于哺乳動物的胎肝,為造血干細胞擴增及分化提供了特定的微環境)為研究對象,利用多重轉錄組測序的方法,繪制了擴增性造血器官的3D轉錄組圖譜,闡釋了造血干細胞和微環境細胞之間的分子調控網絡。

  首先,為了系統性地研究斑馬魚尾部造血組織如何支持造血干細胞擴增、分化的機制,劉峰實驗室利用三種轉錄組測序方法,特定類型群體細胞轉錄組、空間轉錄組和單細胞轉錄組,詳細解析了斑馬魚尾部造血組織的時空特性。不同發育時期特定細胞群體的轉錄組測序分析表明,造血干細胞與相應微環境細胞具有協同發育和共享基因表達的特點。空間轉錄組測序反映細胞位置信息,揭示了多種微環境因素對造血干細胞的不同調控方式,并實現了三維轉錄組重構斑馬魚造血器官。單細胞轉錄組數據更精細闡釋尾部造血組織細胞類群、細胞特征及細胞間分子調控,并發現造血干祖細胞分化和細胞周期存在一定的關聯性。多重轉錄組測序綜合分析揭示了造血干細胞在尾部造血組織的擴增受到內源性和外源性信號的共同調控,鑒定了新的造血干細胞的標記基因,發現了影響造血干細胞擴增的信號通路,完善了造血干細胞-微環境細胞間分子調控網絡。例如,染色質結構維持蛋白smchd1,通過調節造血干細胞增殖促進其擴增;微環境細胞來源的Integrin 信號通路負向調控造血干細胞擴增。同時此項工作還構建了斑馬魚尾部造血組織多重轉錄組數據庫(http://www.picb.ac.cn/hanlab/ichtatlas/Home),可以檢索任何基因在造血組織的表達譜,包括基因表達的時間、細胞類型及相對空間位置。

  該工作不僅發現了造血干細胞特征基因、細胞狀態與細胞分化的關系,闡釋了微環境和造血干細胞互作的調控網絡,更為血液系統研究提供了豐富的數據資源;同時,也將為造血干細胞的體外擴增和造血類器官的體外組裝提供理論指導。該研究論文“A 3D atlas of hematopoietic stem and progenitor cell expansion by multi-dimensional RNA-seq analysis430日在線發表于Cell Reports

  動物所博士研究生薛媛媛和中科院計算生物學研究所博士研究生劉登輝為文章共同第一作者,動物所研究員劉峰和計算生物學所研究員韓敬東為文章共同通訊作者。中科院生物化學與細胞生物學研究所研究員景乃禾與中科院廣州生物醫藥與健康研究院研究員彭廣敦在空間轉錄組測序方面提供了大力支持。該研究得到國家自然科學基金委、國家重點基礎研究發展計劃和中科院器官重建與制造戰略性先導科技專項的資助。

 

  圖1:多重轉錄組測序解析斑馬魚造血器官。多重轉錄組測序,包括:特定類型群體細胞轉錄組測序、空間轉錄組測序和單細胞轉錄組測序,揭示斑馬魚尾部造血組織的時空特性。

 

圖2:斑馬魚造血器官轉錄組數據庫 




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

凤凰Vwww.www.fh7557.com www.c83.me | 004676.com | www.601332.com | www.839216.com | www.ex91.com | www.188318.com | www.768665.com | www.052012.com | www.412250.com | www.11508.cc | www.259717.com | www.388617.com | www.994914.com | 914900.com | www.26988.cc | www.970839.com | 577408.com | 348108.com | www.096266.com | 252063.com | www.509679.com | www.909769.com | www.27759.cc | www.z62.club | 457335.com | www.tv22.com | www.362303.com | www.41140.com | www.px63.com | www.530108.com | 大赢家彩票平台www.127192.com | www.9786.in | www.388227.com | 505147.com | www.377572.com | www.101099.com | www.081039.com | www.220992.com | www.70816.com | www.335822.com | www.805401.com | www.2116.net | www.3gz.cc | www.7636.cn | www.7055.xyz | www.591451.com | www.818925.com | www.wn96.com | www.167871.com | 658067.com | www.pj81.cc | www.28db.com | www.ep3.cc | www.cai1228.com | www.5675.top | www.2941.pw | www.1119.org | www.007217.com | www.44660.com | www.08171.com | www.2668.com | QQ彩票www.89894l.com | www.70eu.com | www.579540.com | www.896554.com | 850403.com | www.775.cc | www.530075.com | www.137.cm | www.9891.loan | www.380955.com | www.801564.com | www.854473.com | www.940603.com | www.948097.com | www.69928.cc | www.062647.com | www.rw91.com | www.268063.com | www.392729.com | www.lv6.com | www.656858.com | www.267931.com | www.795818.com | www.280644.com | www.697765.com | 058810.com | www.281433.com | www.549995.com | www.637678.com | 500万彩票www.500cd.com | www.636691.com | www.04io.com | www.054813.com | www.647547.com | www.11345.com | www.720798.com | 971646.com | www.009983.com | www.461633.com | 中国福利彩票www.6364p.com | www.07if.com | www.257028.com | www.754967.com | 576448.com | www.14qy.cc | www.80qa.com | www.o68.cn | www.10070.com | www.3746.me | www.7660.pw | 500万彩票www.www.98528f.com | www.4971.org | www.177834.com | 050767.com | www.860943.com | www.jt97.com | www.405076.com | www.cp9535.com | www.dk19.com | www.0165.site | www.rc36.com | www.316353.com | 170040.com | www.057932.com | www.703861.com | www.9589.biz | www.097684.com | www.463677.com | www.926289.com | 008351.com | www.539744.com | 013855.com | www.7112.cc | www.gb2.com | www.48ui.com | www.388564.com | 500彩票www.50064r.com | www.254897.com | www.760257.com | www.81ag.com | www.931.red | www.257281.com | www.859548.com | www.my40.com | www.53325.com | www.976745.com | www.860509.com | www.27da.com | www.229489.com | www.761962.com | www.20wu.com | www.050472.com | www.933763.com | www.889967.com | www.823322.com | www.836988.com | www.482118.com | www.89549.com | www.52469.com | www.7088.tv | www.062488.com | www.490013.com | www.700386.com | www.888974.com | 89368.com | 365884.com | www.411324.com | www.t48.bid | 950726.com | www.321503.com | www.235710.com | 017845.com | www.56939.cc | www.228336.cc | www.278134.com | www.891246.com | www.67pd.com | www.028345.com | www.49od.com | www.37by.com | www.5216.wang | www.660243.com | 彩票大家乐www.21202v.com | 699599.com | www.8498.cc | www.98ug.com | www.9061.biz | www.1912.xyz | www.290997.com | www.885916.com | www.19jo.com | www.03456.cc | www.821314.com | www.04185.com | www.789297.com | www.2618.space | www.155607.com | www.eu30.com | www.63.gg | www.1206.com | www.554539.com | www.777017.com | 269074.com | www.2258.pw | www.446100.com | 026825.com | www.09zv.com | www.049595.com | www.220835.com | www.365863.cc | www.971540.com | 140036.com | www.58eo.com | www.015724.com | www.184827.com | www.876365.com | www.8218.biz | www.yd73.com | www.a18.wang | www.5535.cn | www.002904.com | 278758.com | www.ky55.cc | www.348006.com | www.12162.com | www.39fc.cc | www.166116.com | www.021246.com | www.554484.com | 874008.com | www.sr05.com | www.548288.com | 大赢家彩票平台www.369062.com | www.60wh.com | www.5834.net | www.83854.com | www.621547.com | www.05750.com | www.049036.com | www.304811.com | www.rx61.com | 466583.com | www.cai8966.com | www.071297.com | www.440159.com | www.993915.com | 024396.com | www.86352.cc | www.299974.com | 495780.com | www.30ih.com | www.648568.cc | 279033.com | www.93842.cc | 9549.biz | www.185119.com | www.967408.com | 539442.com | www.3798.cm | www.94906.com | www.111630.com | www.572366.com | www.822399.com | www.0566.cc | www.131212.com | www.403772.com | www.862954.com | 857679.com | www.699821.com | www.47046.cc | www.748311.com | www.261535.com | www.800774.com | www.789510.com | www.19248.com | www.66111.cc | www.941517.com | 993374.com | 026473.com | www.227333.com | www.269502.com | 674689.com | www.308002.com | www.40341.com | www.566170.com | www.612561.com | www.850335.com | www.73rl.com | www.027623.com | 447227.com | www.228920.com | www.188806.com | www.894339.com | www.8679.org | www.552.cm | www.216590.com | www.193167.com | www.952994.com | www.6714.cc | www.558961.com | www.274545.com | 266127.com | www.26853.cc | www.co97.com | www.39672.cc | www.75499.com | www.382850.com | 472616.com | www.26757.cc | www.122876.com | www.970209.com | 974697.com | 大奖彩票www.djcp234.com | www.050628.com | www.024878.com | www.625223.com | www.zb10.com | www.mk30.com | 789624.com | www.993.in | www.033632.com | www.485001.com | 539073.com | 爱彩www.235387.com | 华为彩票www.915696.com | www.ry6.com | www.873612.com | www.0915.red | www.410.biz | www.109120.com | www.383725.com | www.654479.com | 683082.com | 274616.com | www.885383.com | www.941677.com | www.36782.cc | www.338049.com | www.899105.com | www.039.cm | www.333325.com | www.404366.com | www.5511.cm | www.86835.cc | www.nq77.com | www.63tc.com | www.cp991.com | 029934.com | www.675245.com | www.82zy.com | www.090299.com | www.230046.com | www.743822.com | www.ti85.com | www.980.cx | www.051106.com | www.zn89.com | 大玩家彩票www.88dwj.com | www.083850.com | www.324697.com | 642875.com | www.757093.com | www.152601.com | www.6137.cn | www.8059.loan | www.627952.com | www.664847.com | www.297835.com | www.874965.com | www.497005.com | www.36000.cc | www.208705.com | www.333954.com | www.149939.com | www.991512.com | www.710363.com | www.235214.com | www.109065.com | www.177293.com | www.986087.com | www.93255.cc | www.333700.com | www.914147.com | www.327828.com | www.128374.com | www.958443.com | 019565.com | www.474251.com | www.0522.cn | www.512828.com | www.550467.com | www.529615.com | www.500841.com | www.470369.com | 659184.com | www.53665.cc | www.48vl.com | www.4029.shop | www.3263.cn | www.987633.com | 947128.com | www.72mx.com | www.243204.com | www.719499.com | www.9690.xyz | www.150181.com | www.918905.com | 881542.com | www.40lg.com | www.552533.com | www.971617.com | www.g33.top | www.03xh.com | www.579852.com | www.56999.cc | www.162967.com | www.160190.com | www.208633.com | www.oo33.com | www.052152.com | 979485.com | 835660.com | www.098579.com | www.022851.com | www.523946.com | www.764161.com | www.938348.com | 24945.com | 818合彩www.www.5666hc.com | www.1005.top | www.088403.com | www.456710.com | www.646781.com | 809202.com | 266305.com | www.jh24.com | www.tg04.com | www.111516.com | www.992656.com | www.502653.com | www.111736.com | www.kr74.com | 074935.com | www.658225.com | www.732259.com | 559092.com | www.550438.com | www.752273.com | 爱米彩www.am5432.com | www.x50.site | www.89957.cc | www.292892.com | www.961467.com | 019468.com | www.342244.com | www.77js.cc | www.h16.xyz | www.rf71.com | www.05383.com | www.525435.com | www.978570.com | a74.cc | www.26yk.com | www.cp2816.com | 941006.com | www.3429.com | www.71066.com | www.og08.com | 092568.com | www.233933.com | www.9781.biz | 060799.com | 65nd.com | www.509859.com | www.774858.com | www.kl11.com | www.364008.com | www.976472.com | www.091365.com | www.097810.com | 吉利彩票www.80075c.com | www.985772.com | www.633862.com | www.bx54.com | 886583.cc | 306532.com | www.552588.com | www.95607.cc | www.153075.com | www.bf77.cc | www.122765.com | www.616784.com | 848575.com | www.gh00.com | www.f31.xyz | www.250898.com | 680702.com | www.019574.com | 639257.com | 458993.com | www.596952.com | www.763865.com | www.220119.com | www.8168.cc | www.951241.com | www.fs6.cc | www.946657.com | www.589081.com | www.063215.com | www.pq88.cc | 884006.com | 网易彩票www.061wy.com | www.98279.com | www.344327.com | www.622273.com | www.805533.com | www.857543.com | 376225.com | 906135.com | www.981293.com | www.510996.com | 534407.cc | www.0122.cm | www.016108.com | www.557741.com | www.979792.com | www.966295.com | 539046.com | 814233.com | www.c66.com | www.241267.com | www.35xm.com | www.540270.com | www.503397.com | cai1123.com | www.54oa.com | www.100996.com | www.953622.com | 765377.com | 468128.com | www.05311.com | www.bw06.com | www.ys56.com | www.964192.com | www.282983.com | www.733127.com | www.249442.com | www.339929.cc | www.870200.com | www.iu32.com | 168320.com | www.a63.cn | 80yv.com | www.7122.pw | www.066571.com | www.068772.com | www.795554.com | 810680.com | www.6550.org | www.581397.com | www.wp59.com | www.47zw.com | www.9509.in | 978425.com | www.t66.com | www.378725.com | www.652429.com | www.984748.com | www.774749.com | www.813255.com | 7156.biz | www.521858.com | www.99962.com | 082066.com | www.046647.com | 57648.com | www.mm.cc | www.1079.cm | www.193895.com | www.358498.com | 818合彩www.www.9889hc.com | 430022.cc | www.856503.com | www.963918.com | www.604238.com | www.458252.com | www.yb64.com | www.947162.com | www.405848.com | www.581771.com | www.474996.com | www.942785.com | www.267551.com | www.04nq.com | www.9608.org | www.137639.com | 267433.com | 067982.com | www.jo.cc | www.73qk.com | www.36438.com | www.25888.cc | www.471400.com | 340575.com | www.578573.com | www.074550.com | www.301283.com | www.166962.com | www.622648.com | www.4er.com | 984691.com | www.861810.com | www.331873.com | www.166365.com | www.254897.com | www.207813.com | www.018491.com | www.7823.loan | www.995921.com | 393131.com | 669647.com | www.86hw.com | www.65rf.com | www.7748.in | www.x22.net | www.949870.com | 620900.com | www.126941.com | www.863501.com | www.877330.com | www.101.cm | www.071098.com | www.122674.com | www.192038.com | www.800301.com | www.822633.com | www.cz81.com | www.1075.wang | www.86177.cc | www.220449.com | www.887057.com | www.pr47.com | www.vs02.com | www.555534.cc | www.96282.com | 快3彩票www.783090.com | www.76vf.com | www.x80.biz | www.984864.com | www.062604.com | www.202294.com | www.159915.com | www.962812.com | 979476.com | 082393.com | www.443543.com | 799207.com | www.713844.com | www.167591.com | www.409958.com | www.803933.com | 510644.com | 071167.com | www.9328.biz | www.8102.com | 772390.com | www.543563.com | 971232.com | 大赢家彩票www.599260.com | www.501625.com | www.610358.com | www.981348.com | www.939859.com | 111453.com | 755895.com | www.yg19.com | 903418.com | 539474.com | www.do4.cc | www.440813.com | www.790688.com | www.583422.com | www.47216.com | www.57233.cc | www.222281.com | www.235739.com | www.297339.com | www.04381.com | www.608310.com | www.730909.com | 071317.com | 379702.com | 848721.com | www.85dw.com | www.459038.com | www.4757.biz | www.ds79.com | www.95wx.com | www.811280.com | 195977.com | www.03270.com | www.42ij.com | 164021.com | www.046858.com | www.66502.com | www.139437.com | www.350105.com | 网易彩票www.501203.com | www.314732.com | www.876613.com | www.od61.com | www.29bh.com | www.9222.vip | www.120602.com | www.557280.com | www.356042.com | www.67299.com | www.111253.com | www.381461.com | 004817.com | 1591.com | www.54183.com | www.260175.com | www.886806.com | 301001.com | www.35084.com | www.135415.com | www.392137.com | www.907926.com | 天玺彩票www.490780.com | 674422.com | 870445.com | 815145.com | www.03wh.com | www.928888.com | www.931957.com | www.895479.com | www.771461.com | www.778604.com | 856525.com | www.122449.com | www.659749.com | 163601.com | www.979170.com | www.521050.com | 041014.com | www.771126.com | www.00195.com | www.06130.com | www.561819.com | www.891342.com | www.927188.com | www.646146.com | www.439570.com | 769411.com | www.yy9.cc | www.28.net | www.hz26.com | 785617.com | 凤凰彩票www.77803n.com | www.uk18.com | www.cp9318.com | www.485737.com | www.662717.com | www.634008.com | www.50fv.com | www.j18.org | 539222.com--kj55.com | 798438.com | 凤凰彩票www.77802f.com | www.8857.win | www.487866.com | 208371.com | www.5093.top | www.89491.com | www.2988.me | www.9wz.com | www.cp5818.com | www.50va.com | tt87.com | www.388603.com | 488965.com | www.ge10.com | www.547868.com | www.8605.wang | 707704.com | www.781822.com | www.630120.com | www.598861.com | www.303241.com | www.005944.com | 497606.com | www.600984.com | www.97456.com | 500彩票www.50054b.com | 987448.com | cai1228.com | 539314.com | 953074.com | 170564.com | 57967.com | 163551.com | www.805961.com | 243444.com | www.gi79.com | 542765.com | www.862681.com | www.5394.xyz | www.28zw.com | www.45136.com | www.08842.com | www.268276.com | www.728552.com | 746637.com | 500彩票www.314677.com | 彩票坊www.022c.cc | www.02206.com | www.07ux.com | www.496000.com | www.713639.com | www.792237.com | 47hk.cc | www.3hb.com | www.ez69.com | www.99798.com | www.522553.com | www.69964.com | www.023341.com | www.502730.com | www.385191.com | www.401.bid | www.lb13.com | 大赢家彩票www.579411.com | www.dp64.com | 500万彩票www.96386n.com | 814149.com | www.96bu.com | www.330575.com | www.649559.com | www.570777.com | www.34qd.com | www.306234.com | www.05xj.com | 251180.com | www.614699.com | www.996836.com | www.671160.com | www.088039.com | 500彩票www.195237.com | 989149.com | www.lq04.com | 973391.com | www.894600.com | www.181164.com | www.670449.com | 148944.com | www.cp1248.com | www.wg19.com | www.586.cm | www.051885.com | www.782162.com | www.116866.com | www.120861.com | www.478213.com | www.398012.com | www.873672.com | www.5190.vip | www.366715.com | www.983497.com | www.je02.com | www.886510.com | 624217.com | www.102914.com | www.235274.com | www.86pu.com | www.017919.com | www.s55.net | www.867045.com | www.05908.cc | www.h92.club | www.804017.com | 88am.cc | www.941738.com | www.790518.com | www.567421.com | www.3809.vip | www.762667.com | 855828.com | www.431005.com | www.1961.club | www.11lr.com | www.253978.com | www.969426.com | www.520149.com | www.36399.com | 750801.com | www.796122.com | www.370146.com | www.220796.com | www.31395.cc | www.09fa.com | www.356673.com | 福彩天下www.609535.com | www.98lo.com | www.048000.com | www.078987.com | www.131791.com | www.89298.com | www.760665.com | www.807231.com | www.382109.com | www.44801.com | www.34lo.com | 861138.com | www.535547.com | 908123.com | www.dt67.com | www.433969.com | www.63786.cc | www.ip33.com | 316022.com | 014272.com | www.382966.cc | www.279208.com | www.579324.com | www.624299.com | www.902221.com | www.947276.com | www.710920.com | www.505401.com | www.lt30.com | 909228.com | 987302.com | 248722.com | 6282.biz | www.889850.com | www.158094.com | www.vl01.com | www.239656.com | 792603.com | www.205538.com | www.9341.biz | www.658603.com | www.969019.com | www.854318.com | 017837.com | www.980.cm | www.50328.com | www.199894.com | www.150022.com | www.9144.in | www.296111.cc | www.84258.com | www.3017.top | 52488.cc | 952921.com | www.964268.com | www.235479.com | www.xh96.com | 184384.com | www.9569.shop | www.003989.com | www.254546.com | www.17483.com | www.c23.biz | www.57229.cc | www.343858.com | www.558465.com | www.434198.com | www.773320.com | www.704504.com | www.560595.com | 506110.com | www.897791.com | www.358400.com | www.491305.com | www.1880.cm | 386234.com | www.520290.com | www.219466.com | www.cp641.com | www.88wc.com | www.572375.com | www.794911.com | cai4449.com | 438066.com | www.62mm.com | www.951981.com | www.345069.com | 111880.com | www.zv18.com | www.7948.cn | www.188261.com | www.eh50.com | 608554.com | www.5as.com | 909123.com | www.127185.com | www.463062.com | www.bi15.com | 623307.com | www.834.cn | www.551353.com | 071980.com | www.al89.com | www.ap99.com | 427986.com | www.245793.com | www.111199.com | www.3052.win | cai7345.com | www.252375.com | www.944222.com | 940937.com | www.720718.com | www.213953.com | www.2895.com | www.259244.com | www.580528.com | www.344043.com | www.661240.com | 017744.com | 692231.com | www.319844.com | www.090037.com | www.682831.com | 279372.com | www.871674.com | 13e彩票www.371680.com | www.vr47.com | www.35332.com | www.282897.com | www.ka67.com | www.bw69.com | 082283.com | www.fg55.com | www.7991.top | www.70559.com | 373062.com | www.547369.com | 178995.com | www.630310.com | www.952446.com | www.863859.com | www.tv73.com | www.3180.cn | www.965508.com | www.91go.cc | www.58239.cc | www.596053.com | www.558545.com | www.611811.com | www.950722.com | www.582350.com | 044363.com | 56303.cc | www.6754.biz | www.700467.com | www.838995.com | www.120563.com | www.191717.com | www.245339.com | 659541.com | 901761.com | www.751548.com | www.711274.com | www.599979.com | 202508.com | 752244.com | www.816339.com | www.102584.com | www.252995.com | www.436898.com | www.869706.com | www.521493.com | www.38642.com | www.54vd.com | www.020457.com | www.qp33.com | www.7122.pw | www.5462.vip | www.31bf.com | www.512653.com | www.952618.com | www.727616.com | www.819519.com | 易彩网www.99638e.com | www.8361.vip | www.bi64.com | 388999.com | www.ly0.com | www.039950.com | www.72815.com | www.275776.com | www.68uw.com | www.en28.com | www.907309.com | www.e28.top | www.40445.com | www.51922.cc | www.038069.com | www.92123.cc | www.6831.xyz | www.129775.com | www.0927.pw | www.237016.com | www.593950.com | www.880935.com | www.819620.com | www.876794.com | www.659562.com | www.334175.com | www.802588.com | 六福彩票www.66ffv.com | www.637317.com | 729055.com | 028934.com | www.984483.com | www.788328.com | www.638779.com | www.og73.com | 天天中彩票www.969751.com | www.87288.cc | 625944.com | 718512.com | www.984951.com | www.2co.com | www.980854.com | www.326958.com | www.279148.com | 301373.com | www.798072.com | www.321605.com | www.8412.cn | www.no8.com | www.643404.com | www.4050.in | www.219054.com | www.148518.com | www.216488.com | 40uk.com | www.301151.com | www.374569.com | www.665945.com | www.546842.com | www.845875.com | www.12250.com | www.6671.org | 500彩票